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DEPC [Diethyl Pyrocarbonate], 5 Milliliters
DEPC treated water reduces risk of RNA degradation in lab
DEPC [Diethyl Pyrocarbonate], 5 Milliliters

Item #: 56231262

DEPC [Diethyl Pyrocarbonate], 5 Milliliters

Item #: 56231262

AZN 121

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DEPC treated water reduces risk of RNA degradation in lab
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What Stands Out

Purity Assurance
DEPC is guaranteed to be of high purity, ideal for researchers requiring reliable reagents for molecular biology applications, enhancing the accuracy of experimental results.
Convenient Packaging
Offered in a 5-milliliter bottle for ease of use and storage, this compact size minimizes waste while providing sufficient quantity for various experiments.
Versatile Usage
DEPC effectively inactivates RNases, making it a crucial agent for nucleic acid research, ensuring the integrity of samples in diverse laboratory procedures.

Product Details

Shop for DEPC [Diethyl Pyrocarbonate] 5 Milliliters at Ubuy Azerbaijan. Modification reagent for proteins. Inhibitor of nucleases. DEPC treated water for RNA handling.
  • Sold for research or further manufacturing use only, not for food or drug use
  • Chemical Formula: C6H10O5
  • CAS Number: 1609-47-8
  • Molecular Weight: 162.1
  • Common Name: Diethyl Pyrocarbonate
  • Modification reagent for His and Tyr residues in proteins. Inhibitor of nucleases. DEPC treated water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases
Item FormLiquid
Item Weight27.22 g
Volume5 Milliliters
Brand NameRPI
ManufacturerRPI, Corp.
Part NumberD43060-5.0
BrandRPI
Item Volume5 Milliliters

Who Should Buy?

Suitable For
  • Molecular Biologists

    Ideal for researchers needing a reliable reagent for RNA and DNA handling applications in molecular biology protocols.

  • Laboratory Technicians

    Useful for technicians performing experiments that require high purity and consistent results in sample processing.

  • Analytical Chemists

    Benefits chemists needing to protect nucleotides and ensure accurate chemical analysis in their lab work.

Not Suitable For
  • Casual Users

    Not suitable for individuals without chemistry background, as improper handling could lead to safety issues.

  • Home Experimenters

    Not advisable for home use due to potential hazards associated with handling chemicals without proper safety protocols.

  • Non-Technical Fields

    Unsuitable for users in non-scientific sectors who do not require complex chemical applications in their work.

Product Description

Have any Query? Chat with us

Customer Questions & Answers

  • Question: What is DEPC [Diethyl Pyrocarbonate] used for?

    Answer: DEPC is commonly used as a reagent in molecular biology and biochemistry. It’s primarily utilized to modify RNA to prevent degradation by RNases, making it essential for experiments involving RNA isolation or analysis. By treating solutions with DEPC, researchers can enhance the reliability of their results, especially in experiments where RNA integrity is critical.
  • Question: How should DEPC be stored?

    Answer: DEPC should be stored in a cool, dry place away from light. Ideally, it should be kept at temperatures between 2-8°C to maintain its effectiveness. Proper storage conditions prevent the degradation of DEPC, ensuring that it remains a potent agent for modifying RNA in your experiments over time.
  • Question: Is DEPC safe to handle?

    Answer: While DEPC is useful in labs, it is important to handle it with care. It is considered hazardous; therefore, using appropriate personal protective equipment (PPE) such as gloves and goggles is recommended. Additionally, ensure proper ventilation in your working area to minimize exposure to fumes, as DEPC can be irritating to the respiratory system.
  • Question: Can DEPC be used with other reagents?

    Answer: Yes, DEPC can be used with various other reagents in molecular biology protocols. However, it is important to add DEPC to solutions before other reagents to avoid potential interactions that could affect the outcome of your experiment. Familiarize yourself with specific protocols where DEPC is indicated for optimal results.
  • Question: How long does DEPC remain effective?

    Answer: The effectiveness of DEPC can depend on storage conditions and exposure to moisture. Generally, when stored properly, DEPC retains its properties for an extended period. It's advisable to regularly check for any changes in appearance or consistency, as these may indicate degradation.
  • Question: What are the effects of incorrectly using DEPC?

    Answer: Incorrect use of DEPC can lead to contamination of your samples, which could compromise experimental results. For instance, if DEPC is not fully hydrolyzed, it can result in the presence of residual diethyl carbonate that may interfere with downstream applications. Familiarity with proper dilution and application methods minimizes these risks.
  • Question: Can DEPC modify DNA?

    Answer: DEPC is primarily designed for RNA modification and does not affect DNA in the same way due to structural differences. While there may be some applications where DEPC interacts with DNA, it is not commonly used for this purpose. For DNA experiments, alternative reagents are preferred to maintain fidelity.
  • Question: How do you prepare solutions using DEPC?

    Answer: To prepare solutions with DEPC, first, dissolve the DEPC in an appropriate solvent, commonly water. It's critical to allow DEPC to react for a minimum of two hours, followed by autoclaving the solution to remove any excess DEPC and hydrolysis by-products that could interfere with your research. Following these steps ensures optimal outcomes in your experiments.
  • Question: What precautions should be taken when disposing of DEPC?

    Answer: Disposal of DEPC should be handled according to local regulations due to its hazardous nature. It’s recommended to neutralize DEPC before disposal, typically by hydrolyzing it with water to convert it into less harmful substances. Always check your institution’s waste disposal guidelines to ensure compliance and safety.
  • Question: Where can I buy DEPC [Diethyl Pyrocarbonate], 5 Milliliters?

    Answer: You can buy DEPC [Diethyl Pyrocarbonate], 5 Milliliters from Ubuy. They offer a wide range of chemicals and laboratory supplies, making it a convenient option for researchers looking to purchase high-quality reagents. Ubuy’s services can help you find what you need to support your biological experiments effectively.

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Pros

  • Effective DNA preservation
  • Wide range of applications
  • Easy handling and storage
  • High purity and quality
  • Cost-effective for laboratories

Cons

  • Requires careful handling

Product Price History

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