DEPC [Diethyl Pyrocarbonate], 5 Milliliters
AZN 121
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from US
QTY:
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
DEPC treated water reduces risk of RNA degradation in lab
Fast
Shipping
Free
Return*
Secure Packaging
100% Original Products
PCI DSS Compliance
ISO 27001 Certified
What Stands Out
Product Details
- Sold for research or further manufacturing use only, not for food or drug use
- Chemical Formula: C6H10O5
- CAS Number: 1609-47-8
- Molecular Weight: 162.1
- Common Name: Diethyl Pyrocarbonate
- Modification reagent for His and Tyr residues in proteins. Inhibitor of nucleases. DEPC treated water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases
| Item Form | Liquid |
| Item Weight | 27.22 g |
| Volume | 5 Milliliters |
| Brand Name | RPI |
| Manufacturer | RPI, Corp. |
| Part Number | D43060-5.0 |
| Brand | RPI |
| Item Volume | 5 Milliliters |
Who Should Buy?
-
Molecular Biologists
Ideal for researchers needing a reliable reagent for RNA and DNA handling applications in molecular biology protocols.
-
Laboratory Technicians
Useful for technicians performing experiments that require high purity and consistent results in sample processing.
-
Analytical Chemists
Benefits chemists needing to protect nucleotides and ensure accurate chemical analysis in their lab work.
-
Casual Users
Not suitable for individuals without chemistry background, as improper handling could lead to safety issues.
-
Home Experimenters
Not advisable for home use due to potential hazards associated with handling chemicals without proper safety protocols.
-
Non-Technical Fields
Unsuitable for users in non-scientific sectors who do not require complex chemical applications in their work.
Product Description
Customer Questions & Answers
-
Question:
What is DEPC [Diethyl Pyrocarbonate] used for?
Answer: DEPC is commonly used as a reagent in molecular biology and biochemistry. It’s primarily utilized to modify RNA to prevent degradation by RNases, making it essential for experiments involving RNA isolation or analysis. By treating solutions with DEPC, researchers can enhance the reliability of their results, especially in experiments where RNA integrity is critical. -
Question:
How should DEPC be stored?
Answer: DEPC should be stored in a cool, dry place away from light. Ideally, it should be kept at temperatures between 2-8°C to maintain its effectiveness. Proper storage conditions prevent the degradation of DEPC, ensuring that it remains a potent agent for modifying RNA in your experiments over time. -
Question:
Is DEPC safe to handle?
Answer: While DEPC is useful in labs, it is important to handle it with care. It is considered hazardous; therefore, using appropriate personal protective equipment (PPE) such as gloves and goggles is recommended. Additionally, ensure proper ventilation in your working area to minimize exposure to fumes, as DEPC can be irritating to the respiratory system. -
Question:
Can DEPC be used with other reagents?
Answer: Yes, DEPC can be used with various other reagents in molecular biology protocols. However, it is important to add DEPC to solutions before other reagents to avoid potential interactions that could affect the outcome of your experiment. Familiarize yourself with specific protocols where DEPC is indicated for optimal results. -
Question:
How long does DEPC remain effective?
Answer: The effectiveness of DEPC can depend on storage conditions and exposure to moisture. Generally, when stored properly, DEPC retains its properties for an extended period. It's advisable to regularly check for any changes in appearance or consistency, as these may indicate degradation. -
Question:
What are the effects of incorrectly using DEPC?
Answer: Incorrect use of DEPC can lead to contamination of your samples, which could compromise experimental results. For instance, if DEPC is not fully hydrolyzed, it can result in the presence of residual diethyl carbonate that may interfere with downstream applications. Familiarity with proper dilution and application methods minimizes these risks. -
Question:
Can DEPC modify DNA?
Answer: DEPC is primarily designed for RNA modification and does not affect DNA in the same way due to structural differences. While there may be some applications where DEPC interacts with DNA, it is not commonly used for this purpose. For DNA experiments, alternative reagents are preferred to maintain fidelity. -
Question:
How do you prepare solutions using DEPC?
Answer: To prepare solutions with DEPC, first, dissolve the DEPC in an appropriate solvent, commonly water. It's critical to allow DEPC to react for a minimum of two hours, followed by autoclaving the solution to remove any excess DEPC and hydrolysis by-products that could interfere with your research. Following these steps ensures optimal outcomes in your experiments. -
Question:
What precautions should be taken when disposing of DEPC?
Answer: Disposal of DEPC should be handled according to local regulations due to its hazardous nature. It’s recommended to neutralize DEPC before disposal, typically by hydrolyzing it with water to convert it into less harmful substances. Always check your institution’s waste disposal guidelines to ensure compliance and safety. -
Question:
Where can I buy DEPC [Diethyl Pyrocarbonate], 5 Milliliters?
Answer: You can buy DEPC [Diethyl Pyrocarbonate], 5 Milliliters from Ubuy. They offer a wide range of chemicals and laboratory supplies, making it a convenient option for researchers looking to purchase high-quality reagents. Ubuy’s services can help you find what you need to support your biological experiments effectively.
RPI Analytical Reagents Editorial Review
Customer Reviews & Ratings
-
5 Star
100%
-
4 Star
0%
-
3 Star
0%
-
2 Star
0%
-
1 Star
0%
Review this product
Share your thoughts with other customers
Pros
- Effective DNA preservation
- Wide range of applications
- Easy handling and storage
- High purity and quality
- Cost-effective for laboratories
Cons
- Requires careful handling
Product Price History
Important information
- Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
- Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.
AZN 121
Order now and get it around Wednesday, July 01
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Features & Benefits
- Modification reagent for His and Tyr residues in proteins
- Inhibitor of nucleases
- Product is for research or further manufacturing use only
![DEPC [Diethyl Pyrocarbonate], 5 Milliliters](https://m.media-amazon.com/images/I/417BxA2Zm7S._SS100_.jpg)